Sierra Leone health ministry spokesman Sidi Yahyah Tunis announced on Thursday a second Ebola reported case affecting someone closely linked to the last person to contract the virus.
Nearly 150 of the original victim's contacts - 50 at high risk of contagion - have been taken under supervision, and the new patient is being kept under quarantine.
Mariatu Jalloh, aged 22, died in mid-January and officials allowed her family organise a traditional funeral.
During traditional funerals many mourners touch the victim’s body, likely the key reason for the latest outbreak of the virus.
World Health Organization officials have said new cases could emerge.
The Ebola virus has killed at least 11,000 people since 2013.