Zeki Alasya had been receiving treatment at the Koç University Hospital since April 22.
His long term comedy partner Metin Akpinar said "I have lost my other half. My heart is aching. We were never separated until now. He will never be forgotten."
His career started in 1959 at the theater of a students union. He directed as well as acted in countless plays and movies.
He was also one of the founders of the Devekuşu Kabare (Ostrich Cabaret) Theater along with Metin Akpinar. The duo shaped the cabaret culture in Turkey in the 80s.
Atilla Dorsay, a Turkish movie critic, told local media that: "Alasya was one of the most prominent comedians of modern times."
Alasya received the prestigious "State Artist" title in 1998 and was granted a "Lifetime Honor Award" in 2010 by the International Antalya Golden Orange Film Festival.
His funeral will be held in Istanbul Levent Mosque on Sunday.