Turkish F-16 fighter jets departed from Turkey's 2nd Air Force Command, 8th Main Jet Base located in Diyarbakir hit positions used by the outlawed PKK and ISIS in Northern Iraq and Syria respectively on Friday evening.
Prime Minister Ahmet Davutoglu held a late-hour emergency meeting in the Cankaya Palace in Ankara about the operation against ISIS and the PKK.
Several ministers and senior army officials attend the meeting including Interior Minister Sebahattin Ozturk, deputie of PMs Yalcin Akdogan and Bulent Arinc, former interior minister Efkan Ala, National Intelligence Organisation Chief Hakan Fidan, Commander of the Air Force General Akin Ozturk and Commander of the Turkish Gendarmerie Forces General Abdullah Atay.
The Prime Ministry released a statement saying that the operation targeted PKK camps, shelters, caves and logistics points in Zap, Metina, Gara, Avasin-Basyan, Sinath Haftanin, Hakurk and Qandil which are located in the mountainous northern Iraq region.
The Second air operation by the Turkish Air Force in the last 24 hours, the air strikes reportedly started around 11:00pm local time (2000 GMT).
The Ministry also noted they informed their international allies, including NATO and the United Nations, about the military operation.
In the operation which took place early Friday, Turkish F-16 jets hit ISIS targets in Syria from inside Turkey, refraining from entering Syrian airspace.
Turkey has stepped up efforts against ISIS since a suicide attack on Monday allegedly carried out by the group killed 32 people in the southeastern Sanliurfa province.
Two policemen were killed in retaliation on Wednesday. The PKK claimed responsibility for that attack.
On Thursday, gunfire from Syria killed a Turkish military officer and injured two sergeants in the southern Kilis province, prompting Turkish forces to return fire.