The same region experienced four earthquakes in the last six weeks. The one on August 24, measuring 6.6, killed nearly 300 people.
A 5.0 magnitude earthquake rocked central Italy on Thursday morning, in the same region that was struck by recent strong quakes, the US Geological Survey (USGS), said.
Two quakes measuring 5.5 and 6.1 struck the area on October 26, followed by a 6.6 magnitude earthquake on Sunday, the biggest temblor to strike Italy for 36 years. In August, a 6.2 earthquake in the same region killed nearly 300 people.
The USGS said that Thursday's quake was very shallow, only 10 kilometres (6.2 miles) deep, and was centred 51.5 km (32 miles) southeast of Perugia.
The shallow depth would have intensified the shaking at the surface.
5.0 earthquake, 4km SW of Pieve Torina, Italy. 2016-11-03 01:35:02 at epicenter (21m ago, depth 10km). https://t.co/6vL43UrBLC— Earthquakes Tsunamis (@NewEarthquake) November 3, 2016
A 5.0 magnitude earthquake is considered moderate and is capable of causing considerable damage.
However, local media said no fresh collapses or emergencies had been reported.
Sunday's 6.6 magnitude earthquake unleashed widespread damage, levelling historic towns and villages in the Apennine mountains and displacing 15,000 people.
Italy sits on two fault lines, making it one of the most seismically active countries in Europe.