The graffiti was removed due to the company's anti-graffiti policy but a Transport for London spokesman said, "We appreciate the sentiment of encouraging people to wear face coverings".
British street artist Banksy's latest work, tackling the spread of coronavirus, that appeared inside a London Underground train carriage "some days ago" has been removed, according to transport operators.
The graffiti star uploaded a video on social media on Tuesday, captioned "If you don't mask, you don't get".
In it, the artist is seen in a boiler suit spray painting stencils of his iconic rats inside a train, with one sneezing blue droplets.
Transport for London (TfL) later told AFP the work was removed "some days ago" due to the company's strict anti-graffiti policy.
I know @TfL have a rule on graffiti removal, but I’d have like this urban art by #Banksy to have remained for a good number of weeks. Especially as it carries a message in these bizarre & challeneging times#MaskMoaners #NoMask #urbanart #londonunderground #tube #coronavirusuk pic.twitter.com/7hSxM6eDXs— Nic (@N1ngN1ng) July 15, 2020
However, the transport operator would welcome Banksy to recreate his message "in a suitable location", it added.
"We appreciate the sentiment of encouraging people to wear face coverings, which the vast majority of customers on our transport network are doing," a TfL spokesman said.