The Nobel laureate, who was ousted from power in a 2021 coup, has been charged with at least 18 offences, which carry combined maximum jail terms of more than 100 years if found guilty.
Some 1,619 prisoners, including 42 foreigners to be "pardoned" and will be released to mark the Buddhist new year, Myanmar's junta has said.
Army chief Min Aung Hlaing remarks came as the anti-coup protesters called for a national "power strike" demonstration on the anniversary of the bloodiest crackdown so far on democracy protests.
The measures come as Washington increasingly punishes the military both for the February 2021 coup that ousted Aung San Suu Kyi and violence against the Muslim Rohingya minority.
The increased indiscriminate attacks by Myanmar's military in the country's eastern region have killed and displaced tens of thousands with air attacks constituting "war crimes."
The ousted lawmakers from the Asian nation's "National Unity Government" were targeted for violating "the existing laws" and "committing acts that could harm the interests of Myanmar".
The bloc says it's deeply concerned by the continuing escalation of violence in Myanmar.
Military rule in Myanmar has announced that the amnesty will be placed on the nation’s Union Day.
Opponents of the military rule have marked the one-year anniversary of the army’s seizure of power with a nationwide strike to show their strength and solidarity amid international concerns.
Arrests followed official warnings that anyone taking part in silent strikes would be liable to prosecution on at least four counts, including under the Counterterrorism Law.
Activists, Ko Jimmy and Maung Kyaw have been detained since their arrests, unable to comment on the allegations, and no lawyer ever emerged to comment on their behalf.
The United Nations estimates almost 90,000 people have fled after intense fighting in Loikaw town in eastern Myanmar's Kayah state last week.
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