Swiss diplomat Pierre Krahenbuhl, who headed the United Nations Relief and Works Agency for Palestine Refugees, is facing allegations of management misconduct.
As they commemorate the Nakba, or 'catastrophe', Palestinians see the UNRWA as a vital ingredient in the struggle for freedom, justice and equality.
This is the first formal appeal broadcast on official media. The regime recently retook large areas near Damascus, and are waging a new offensive in the south that UN officials say has displaced more than 270,000 people.
State Department spokeswoman Heather Nauert says the US cannot be expected to be "the chief donor to every organisation around the world".
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