Houthi and medical officials say the detention centre was holding at least 170 people when it was hit.
Talks between Saudi-backed government and UAE-backed separatist rebels reach a dead-end as both sides gather troops to prepare for further battles deepening rift between Riyadh and its ally Abu Dhabi.
A Saudi-led coalition air strike on a prison in Yemen has reportedly killed more than 100 people. The coalition insists its target was a military site. #Yemen #Saudi
UN war crimes investigators said an independent panel sent a secret list to UN human rights chief Michelle Bachelet identifying "individuals who may be responsible for international crimes" in the country.
Saudi-led coalition air strike was carried out in the city of Dhamar, south of Yemen's capital Sanaa. Rebel spokesman Mohamed Abdul-Salam said a detention centre was hit but the coalition claimed it targeted a "military site".
In a rare statement, President Abed Rabbo Mansour Hadi condemned the UAE, a former ally, for "blatant intervention" in Yemen by providing "support, money and plans" for separatists who aim at "dividing up" the country.
As the Saudi-led Gulf coalition is fractured by the withdrawal of the UAE from Yemen, Washington seeks an exit strategy to end its engagement in the country.
Residents said fighting restarted in Aden when government forces attacked the Yemeni city's eastern suburbs and fought artillery duels with UAE-backed southern separatists.
The move comes after the United Arab Emirates-backed Shabwani Elite forces withdrew from the area.
Faced with funding shortfalls, the UN has already ended a vaccination campaign and scrapped plans for nutrition centres, shelters and mental health clinics and has shuttered a water purification plant for farmers.
Meanwhile, Human Rights Watch says the Saudi-led coalition carried out at least five deadly attacks on Yemeni fishing boats in 2018, killing at least 47 Yemeni fishermen, including seven children.
The Gulf allies are backing two different sides adding another layer of complexity to year-long conflict.
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