"Evacuating the observation post in Idlib after the regime's attack is definitely not happening, it won't happen anywhere," Turkish Defence Minister Hulusi Akar said.
Turkey already proposed to form a joint working group to assess whether the S-400 will pose a threat to NATO or not, said Foreign Minister Mevlut Cavusoglu.
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Tensions between US and Turkey have worsened in recent months with Turkey set to begin receiving the S-400 Russian surface-to-air missile system, which the US says will jeopardise Ankara's role in the F-35 fighter jet programme.
The US move to prohibit Turkey from acquiring F-35 jets can be a major setback to NATO, as other world powers race to catch up.
Remarks by Defense Department spokesman are in contrast to comments made earlier in the day by Senator Jim Inhofe who said a decision was made to stop training the Turkish pilots.
Acting US Defense Secretary Patrick Shanahan's comments came a day after the Pentagon suspended the delivery of equipment related to the F-35 fighter jet to Ankara.
The Pentagon also has started to look at secondary sources to produce parts for F-35s that were being developed in Turkey.
"Despite some statements, the process of procuring F-35 jets is working fine. Our pilots and maintenance team continue training in the US," - Turkish Defebce Minister Hulusi Akar.
Turkish President Recep Tayyip Erdogan says the pressure against Ankara's purchase of S-400 air defence system is because "Turkey takes action with its own will regarding the regional developments, particularly Syria."
Washington has cautioned that the S-400 system might covertly obtain critical information on the advanced fighter jets, including their detection range, which could then be relayed to Russia.
The Pentagon says it will reassess the promised delivery of F-35s to Turkey, but the report doesn't address Ankara's major concerns.
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