Palestine Red Crescent said eleven of the injured were taken to a hospital and most injuries occurred from the police's use of plastic bullets and gas bombs on unarmed Palestinians.
Israeli police have used stun grenades, batons and rubber bullets to prevent Palestinians from reaching the Al Aqsa Mosque for prayers on Islam’s holiest night of Laylat al Qadr, sparking outrage across the Muslim world.
Despite international condemnation, the Israeli police, some of them on horseback and backed by water cannons on Saturday, used force and barricades on thousands of Arab Muslims who see East Jerusalem as a capital of their future state.
What is happening to the Palestinian people is heartbreaking and inhumane! How could any human being defend this disgusting behaviour?! How could they kick people out their homes?! 💔 #PrayForPalestine #Jerusalem https://t.co/UjgrSrAuTs— Dalida (@DalidaDavid85) May 9, 2021
'Israelis do not want us to pray'
At least 80 people were injured, including minors and a one-year-old, and 14 were taken to hospital, the Palestine Red Crescent said. Israeli police said at least one officer was hurt.
"They do not want us to pray. There is a fight every day, every day there are clashes. Every day there are troubles," said Mahmoud al Marbua, 27, speaking near the Old City's Damascus Gate. Pointing to police chasing youths and firing thunder-flashes at them, he added: "Look how they are firing at us. How can we live?"
Tensions have mounted in the city throughout the Muslim holy month of Ramadan, amid growing anger over the potential eviction of Palestinians from Jerusalem homes on land claimed by Jewish settlers.
In Gaza, hundreds of protesters gathered along the border with Israel. The Israeli forces said the crowds threw burning tyres and firecrackers toward the troops.
Hamas fired at least one rocket into Israel which landed in an open area, the military claimed.
"We salute the ppl. of Al Aqsa, who oppose the arrogance of the Zionists & we call on our ppl. in Palestine to support their brothers by all means," Moussa Abu Marzouk, a leader of the armed group Hamas that runs Gaza, said on Twitter.
A group of right wing #Israeli activists have attacked 3 Palestinians near Jaffa Street, West of J'lem. #Police have arrived on scene.pic.twitter.com/khrtvpyrmF#فلسطين #القدس_تنتفض #Israel #Jerusalem #Palestine #News #MiddleEast #عاجل— Andrew Jose (@realandrewjose) May 9, 2021
Israel said it was beefing up security forces on Saturday in anticipation of further confrontations in Jerusalem, the occupied West Bank, and Gaza after fierce clashes erupted the previous night at Al Aqsa Mosque.
A Palestinian official said Egypt was mediating between the sides to prevent further escalation and Saturday's violence appeared less pronounced than Friday's events.
On Friday, police fired rubber bullets and stun grenades towards rock-hurling Palestinian youths at the mosque on the Noble Sanctuary/Temple Mount plaza holy to both Muslims and Jews.
At least 205 Palestinians and 18 Israeli officers were injured in Friday's confrontations, which drew international condemnations and calls for calm.
Clashes have erupted nightly in East Jerusalem's Sheikh Jarrah - a neighbourhood where numerous Palestinian families face eviction in a long-running legal case.
Israel said dozens of protesters threw stones at officers there on Saturday.
Police Commissioner Yaakov Shabtai said extra officers were being deployed in Jerusalem on Saturday to "enable freedom of worship and maintain order and security."
"At the same time, we will not allow violent riots, lawbreaking or the harming of police officers. We ask everyone to calm the spirits and violence, particularly on such an important day for the Muslim religion," Shabtai said in a statement.
The Israeli forces said it was boosting troops in the occupied West Bank and near Gaza, where Palestinians have sent incendiary balloons over the border.
Thousands strong at Al Aqsa tonight despite Israel’s bullets, stun grenades, arrests and checkpoints. The Palestinian spirit cannot be crushed. pic.twitter.com/RDzbV0eq8x— IMEU (@theIMEU) May 8, 2021
The Middle East Quartet of mediators - the United States, Russia, the European Union, and the United Nations expressed concern over the violence and potential Jerusalem evictions.
"We call upon Israeli authorities to exercise restraint and to avoid measures that would further escalate the situation during this period of Muslim Holy Days," the Quartet said in a statement.
Prime Minister Benjamin Netanyahu said in a statement that law and order would be maintained in Jerusalem as would the right to worship.
Television footage showed buses of Muslim worshippers heading from Israeli Arab cities to Al Aqsa being stopped by police on the main highway to Jerusalem.
Word of the roadblock spread on social media, drawing hundreds of young men from nearby Arab villages and from Jerusalem.
'We will redeem you, Al Aqsa!'
Dozens drove their cars the wrong way down the now-empty Jerusalem-bound lanes, picking up fellow Muslims who had abandoned their own vehicles to start the uphill trek on foot. Some chanted in Arabic: "With our souls and our blood, we will redeem you, Al Aqsa!"
Police said it was stopping only those planning to take part in riots before the buses were allowed to proceed.
Scuffles broke out and footage showed officers firing stun grenades.
Tension was expected to remain high over the next few days. Israel's Supreme Court will hold a hearing on the Sheikh Jarrah evictions on Monday, the same day that Israel marks Jerusalem Day - its annual celebration of its capture of East Jerusalem during the 1967 Middle East war.
Palestinians want East Jerusalem as the capital of a future state. Israel claims the entire city as its eternal, indivisible capital. Its annexation of the eastern section was not recognised internationally.