Both sides will clash in England's Headingley as ties between Islamabad and Kabul remain frosty with both accusing each other of patronising militants.
Afghanistan versus Pakistan is perhaps the World Cup match that cricket fans in the war-weary nation will be watching most intensely.
It is a complicated relationship.
Many Afghanistan players learned the game growing up as refugees in Pakistan but lots of Afghans accuse their powerful neighbour of supporting Taliban insurgency. And Islamabad believes Afghanistan is being used by Kabul-backed militants and rival India to destabilise its western border.
With years of distrust and blame between the two countries, a high-stakes victory over Pakistan in the sport it loves best on Saturday would taste especially sweet to Afghans.
There is also intrigue on the pitch at Headingley, in the northern English city of Leeds.
Pakistan cannot afford any more slip-ups, needing to win their final two matches to retain a realistic chance of reaching the semi-finals after a slow start to the tournament.
Afghanistan, appearing in just their second World Cup since their debut in 2015, are playing for pride after losing all seven of their matches so far.
Pakistan will beat Afghanistan in tomorrow's match and reach the semis.— Shoaib Akhtar (@shoaib100mph) June 28, 2019
Crucial time for Pakistan to continue the winning streak. #CWC19 #PAKvsAFG #PakvsInd
Watch the full video here: https://t.co/vMoN8T9bci pic.twitter.com/iSoWdNAoFG
'We learnt a lot of cricket in Pakistan'
Afghanistan captain Gulbadin Naib played down the political tensions between the two nations at his pre-match press conference on Friday, saying he hoped cricket could build bridges.
"If you look at our cricket, we learnt a lot of cricket in Pakistan and we also played cricket in Pakistan," Naib said.
"We are also hoping cricket is one thing that can keep a good relationship for anyone, any other country. So I am hoping that not only with Pakistan, but with India, Sri Lanka and other countries."
Cricket became popular in Afghanistan after the Soviet invasion of 1979, with young people learning the sport in refugee camps on the Pakistan border.
All-rounder Mohammad Nabi and leg-spinner Rashid Khan started playing the game in Peshawar while the Pakistan Cricket Board helped Afghanistan cricket by providing equipment and playing opportunities.
Two former Pakistan internationals – Kabir Khan and Rashid Latif – have played key roles in the development of Afghanistan as coaches over the past decade.
But the lingering tensions mean that Afghan players are reluctant to draw attention to their association with Pakistan and often show more of an affiliation with Pakistan's arch-rivals, India.
On the pitch, Afghanistan beat their formidable rivals in a pre-World Cup warm-up match, but they have lost all three official one-day internationals between the two sides.
'Afghanistan can be dangerous'
Despite this winning record, Pakistan skipper Sarfaraz Ahmed warned his players not to take Afghanistan lightly.
"We know they have quality spinners in their team," said Sarfaraz, referring to Rashid, Nabi and Mujeeb Ur Rahman. "We can't take them lightly because a team such as Afghanistan can be dangerous."
"We have staged a comeback with a strong belief that we can win and then our way to the semi-final is paved, but at the moment we have only Saturday's game on our minds," he added.
Pakistan's last match of the round-robin phase is against Bangladesh, which could turn into another virtual quarter-final at Lord's on July 5 as they target the semi-finals and dream of a second World Cup triumph.
Afghanistan's sights are set lower but they have a long-term dream.
Naib said he hoped playing at tournaments such as the World Cup would boost the standard of the game in Afghanistan.
"We want more matches to build our cricket so it's really good for us to play a lot of cricket with them (major nations)," said the Afghan skipper.