Marie Yovanovitch testifies in the second public hearing even as US president launches Twitter attack on her during the testimony, dubbed "witness intimidation" by Democrats.
The first open impeachment hearings into US President Donald Trump will begin next week as the investigation heads into a highly anticipated public phase.
A released testimony revealed Gordon Sondland, a top ally of the US president, admitted he told a Ukraine official that US military aid was contingent on Kiev investigating Donald Trump's Democratic rival Joe Biden.
Gordon Sondland had been due to meet behind closed doors with staff of three Democratic-led House committees over the role he may have played in President Trump's efforts to get Ukraine to probe former vice president Joe Biden, a political rival.
US President Donald Trump boiled over at the impeachment inquiry Democrats are pursuing over his July 25 phone call to Ukraine President Volodymyr Zelenskiy.
Democrats ask White House for documents related to President Trump's call with Ukraine leader and subpoena Secretary of State Mike Pompeo, who admits listening in on the July call that's triggered impeachment inquiry of Trump.
US Secretary of State Mike Pompeo accuses Democrats of bullying and intimidation in asking five diplomats to testify on White House efforts to seek dirt on President Trump's political rival.
Russian caveat comes after US House Intelligence Committee vowed to get access to President Trump's calls with Russian President Putin and other world leaders, citing concerns Trump may have jeopardised national security.
US President Donald Trump confirmed he did discuss former vice president Joe Biden and corruption allegations in a phone call with Ukraine's leader.
Special counsel Robert Mueller's report, released publicly in April, found Russia meddled in the 2016 presidential election and that Republican President Donald Trump's election campaign had multiple contacts with Russian officials.
House Intelligence Committee Chairman Adam Schiff says in a statement the subpoenas issued included a “friendly subpoena to Deutsche Bank.”
"We are going to initiate investigations into abuses of power, into corruption and into obstruction of justice," the chairman of the House committee, Rep. Jerrold Nadler, D-N.Y. said. "We will do everything we can to get that evidence."
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